Method of immunoassay of enzymes in the solid phase by application of a single column chromatography

摘要

The present invention is characterized in that, in the immunoassay immunoassay (or) of enzymes in the conventional solid phase, all the steps of the step of immunological reaction of the ingredients has an immunological reaction, or an immunological complex of these products, with a product that react adsorbed on a support, at the step of measuring the activity of the enzyme is contained in the product insolubilizes resultant, are applied by the application of a single column chromatography. The process of the present invention has the advantages, such that a large number of antigens or antibodies may be determines, by using an enzyme which is identical to or an insoluble support identical, this large impact, and this high precision can be obtained without operations are difficult for the process.