HUMAN-HUMAN HYBRIDOMA, PRODUCTION THEREOF AND MONOCLONAL ANTIBODY PRODUCED BY SAID HYBRIDOMA

摘要

PURPOSE:To obtain a human-human hybridoma system capable of producing a monoclonal antibody having low heteroantigenicity, by fusing a transformed human cells subjected to multiplication inhibiting treatment to antibody- producing human cells. CONSTITUTION:Human cells, preferably B cells, hepatic cells, splenic cells, oenocytes, lymphonodus cells, etc., are transformed by viral sensitization, genetic recombination method, etc., to inhibit the multiplication of preferably homozygous type by treatment with emetine and actinomycin D, etc. Antibody- producing human cells, e.g. B cells, plasma cells, hepatic cells, fetal hepatic cells, splenic cells, lymphonodus cells, cultivated B cells, etc., preferably transformed B cells, are subjected to cell fusion to the above-mentioned transformed cells subjected to the multiplication inhibiting treatment in, e.g. RPMI1640 culture medium, etc., by using polyethylene glycol, etc., to give a human-human hybridoma, which is then cloned. Monoclonal antibodies are then produced by using the above-mentioned cloned hybridoma.