MONOCLONAL ANTIBODY AGAINST HUMAN PANCREAS PHOSPHOLIPASE A2, ITS PRODUCTION, HYBRIDOMA PRODUCING SAID MONOCLONAL ANTIBODY AND DETERMINATION OF HUMAN PANCREAS PHOSPHOLIPASE A2 USING SAID MONOCLONAL ANTIBODY

摘要

NEW MATERIAL:An antibody having specificity to human pancreas phospho lipase A2. USE:Reagent for determination of human pancreas phospholipase A2 and useful for the diagnosis of acute pancreatitis, etc. PREPARATION:An experimental animal such as mouse is immunized by using purified human pancreas phospholipase A2 as an immunogen and a B lymphocyte is collected from the immunized animal. The lymphocyte is fused with a myeloma cell of permanent myeloma cell system. The obtained hybridoma is screened e.g. by enzyme immunoassay to select a hybridoma capable of producing the objective antibody. Finally, the hybridoma is monoclonized and the monoclonal cell strain of said hybridoma is cultured in the peritoneal cavity of the animal used in immunization. The objective antibody can be separated from the ascites of the animal.