SEPARATION OF SINGLE STRANDED T-PA AND DOUBLE STRANDED T-PA

摘要

PURPOSE:To separate a single-stranded t-PA and double stranded t-PA, by bringing an aqueous medium containing the single-stranded t-PA and double- stranded t-PA once into contact with a carrier carrying ETI to adsorb the t-PA and changing the pH of the eluate. CONSTITUTION:A cell culture fluid is passed through a column immobilizing STI which is a Kunitz type inhibitor forming soybean seeds different from ETI in that t-PA (tissue plasminogen activator) is not inhibited to remove a proteolytic enzyme of the type bonding to the ETI. The unadsorbed solution is then passed through the ETI column to adsorb the t-PA and impure proteins are washed and removed to elute the above-mentioned t-PA within a pH region where the single stranded t-PA is eluted. Then single stranded t-PA and double- stranded t-PA or double stranded t-PA is eluted within a pH region where the single-stranded t-PA and double stranded t-PA are eluted to carry out separation and purification.