NOVEL STEROID COMPOUND, ITS PREPARATION, AND ANTIGEN FOR PREPARING ANTIBODY FOR MEASURING ESTRONE SULFATE BY IMMUNOCHEMICAL MEASUREMENT COMPRISING IT

摘要

NEW MATERIAL:6alpha-Hydroxy estrone-3-sulfate-6-hemisuccinate-6-BSA bonded compound shown by the formula I (-NH-BSA is bovine serum albumin residue). USE:An antigen for preparing an antibody for measuring estrone sulfate by imminochemical measurement. An amount of estrone sulfate can be determined accurately by immunochemical method, e.g., radioimmunoassay method, enzyme antigen method, hemagglutination method, latex agglomeration method, etc. without cross reactions and an ovulatory day is measured effectively. PROCESS:The OH group of D ring of 6alpha-hydroxy estadiol-6-hemisuccinate shown by the formula II is oxidized, sulfonated, and reacted with N-hydroxy-succinimide or p- nitrophenol in the presence of a condensing agent to give compound shown by the formula III or IV, which is reacted with bovine serum albumin, to give compound shown by the formula I.