METHOD OF PROTEINS EXTRACTION AND CLEANING FROM PENETICALLY CHANGED YEAST CELLS' CULTIVATING MEDIUM WHERE PRODUCTION OF ABOVE-MENTIONED PROTEINS OCCURRED

摘要

The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent; it comprises the precipitation of contaminants by polyethylene glycol and the treatment of this latter supernatant with either a bivalent metal cation or, after eventual ultrafiltration, with ammonium sulfate.