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FAERGMAERKAEMNE FOER KLONERINGAR I STREPTOMYCES LIVIDANS.
FAERGMAERKAEMNE FOER KLONERINGAR I STREPTOMYCES LIVIDANS.
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机译:链霉菌属克隆的分类
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摘要
Subjection of Streptomyces coelicolor DSM 3030 to total digestion of the complete DNA, cloning into a suitable vector and transformation of Streptomyces lividans result in colonies with an intense crimson colour. Reisolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which the gene crucial for pigment production is located. The latter is suitable as marker, in particular as inactivation marker, in Streptomycetes.
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