PROCESS FOR UTILIZING EUCARIOTE EXPRESSIVE VECTORS CONTAINING BK VIRUS INCREASER

摘要

A method for producing a functional polypeptide in a eukaryotic host cell wherein the cell is transformed with a recombinant DNA vector that contains a eukaryotic promoter and a DNA sequence that encodes the functional polypeptide, the sequence being positioned for expression from the promoter, and wherein the cell containing said vector is cultured under conditions suitable for expression of the functional polypeptide, which comprises: (a) incorporating DNA that encodes the first part of the tripartite leader of an adenovirus into said vector such that, upon transcription, the mRNA produced encodes the functional polypeptide and, at the 5' end, contains the first part of the tripartite leader; (b) providing the cell containaing the vector of step a) with a DNA sequence that codes for the expression of a VA gene product of the adenovirus; and (c) culturing the cell of step b) under conditions suitable for expressing the VA gene product and for stimulating translation of the mRNA, subject to the limitation that the mRNA does not contain the entire tripartite leader of the adenovirus.