analytical method impiegante reagents biospecifici marked with colored macromolecules.

• 1. 用于生物大分子标记的带色生物素及其在制备生物大分子标记试剂中的应用 [P] . 中国专利： CN102219791A . 2011-10-19
• 2. 一种蛋白类生物大分子快速偶联标记色素基团试剂盒 [P] . 中国专利： CN106483295A . 2017-03-08
• 3. analytical method impiegante reagneti biospecifici marked with colored macromolecules [P] . IT1217993B . 1990-03-30

  机译： 分析方法用有色大分子标记的抗生物素蛋白

• 4. ANALYTICAL METHOD AND COLORED ANALYTICAL REAGENT FOR PROTEIN FIXATION ASSAYS [P] . 法国专利： FR2626674A1 . 1989-08-04

  机译： 蛋白质固定分析的分析方法和着色分析试剂

• 5. Analytical method using biospecific reagents labelled with colored macromolecular structures [P] . 英国专利： GB2215047A . 1989-09-13

  机译： 使用标记有色大分子结构的生物特异性试剂的分析方法

• 1. A marked enhancement in production of amylase by Bacillus amyloliquefaciens in flask fermentation using statistical methods [J] . ZHAO Wei, ZHENG Jia, WANG Yu-guang, 中南大学学报（英文版） . 2011,第004期
• 2. Total phenolic, flavonoid content, and antioxidant activity of flour, noodles, and steamed bread made from different colored wheat grains by three milling methods [J] . Yaoguang, Li, Dongyun, 作物学报：英文版 . 2015,第004期
• 3. SPIN-MARKED REAGENTS IN FLOTATION [C] . P. Solozhenkin, V. Nebera, V. Latin XXI International Mineral Processing Congress . 2000
• 4. Enhanced Sampling Methods for the Computation of Conformational Kinetics in Macromolecules. [D] . Grazioli, Gianmarc. 2016
• 5. A Novel Analytical Method for Trace Ammonium in Freshwater and Seawater Using 4-Methoxyphthalaldehyde as Fluorescent Reagent [O] . Ying Liang, Yingming Pan, Qing Guo, 2015
• 6. Validation of an Analytical Method to Determine the Content of Sucralose in Beverages - Report on a Method Validation by Collaborative Study Determination of Sucralose in Beverages by Thin Layer Chromatography in Combination with Reagent-free Derivatisation and Ultraviolet/Fluorescence Detection [O] . DONCHEVA TSANEVA IVANKA, STROKA JOERG 2007
• 7. Towards a Methodology for Breaking Carbon-Carbon Bonds in Coal Macromolecules. Final Report January 1, 1985-December 31, 1987 [R] . Arnett, E. M. 1988

• 1. Preparation and Spectrophotometric Study of Reagent 2-2-（6-chloro benzothiazolyl） azo-Resorcinol as an Analytical Reagent for Determination of Nickel （11） and Copper （11） Ions [J] . Khalidj. AL-Adilee ,Azhar-Ghali ,Ashwoq S. Hussein . 化学与化工 . 2012,第007期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. A marked enhancement in production of amylase by Bacillus amyloliquefaciens in flask fermentation using statistical methods [J] . 赵伟 ,郑甲 ,王玉光 . 中南大学学报 . 2011,第004期
• 4. 游离前列腺特异性抗原定量标记免疫分析试剂盒行业标准建立 [C] . 黄颖 . 中国环境诱变剂学会风险评价专业委员会全国第十三届学术会议暨第四届第5次委员会议——低碳生活与健康损害论坛 . 2011
• 5. 新型荧光试剂2，5-二-[2-（4-羟基-苯基）-乙烯基]吡嗪及其衍生物用于生物大分子识别 [A] . 谢飞燕 . 2007