METHOD FOR GROWTH AND PURIFICATION OF HUMAN CELL LINES FROM HETEROGENEOUS CELL POPULATIONS IN TISSUE CULTURE

摘要

SK 284ABSTRACTHuman melanocyte cells are grown in tissue culturemedium containing fibroblast growth suppressor plus tumorgrowth promoters at pH 7.2 to about 7.4. In a stepwiseprocedure, Langerhans, keratinocytes and most fibroblastsare removed in the presence of the above suppressor andpromoter followed by a subsequent trypsinization treatment.In a final purification step, remaining fibroblasts areseparated from the melanocytes by reacting the cell mixturewith monoclonal antibody specific for melanocytes, and thenseparating out the melanocytes from the fibroblasts ondensity gradients.