Expression vectors containing the heat shock regulatory DNA sequences from a heat shock protein 83 (hsp83) gene and inducible expression by the use of the vectors

摘要

Expression vectors are constructed in which a desired foreign structural gene which encode, for example, physiological active substance such as human tissue plasminogen activator is functionally linked to heat shock regulatory DNA sequences from Drosophilamelanogaster approximately 83 k daltons heat shock protein (hsp83) gene or the modified DNA sequences of the regulatory DNA sequences and other DNA sequences necessary for translation of the desired foreign struc­tural gene.;Cells transformed with the expression vector are grown and the production of the desired structural gene products by the transformed cells is induced by heat shock treatment when necessary.