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Process and medium for culturing ant venom gland cells
Process and medium for culturing ant venom gland cells
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机译:培养蚂蚁毒腺细胞的方法和培养基
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摘要
In vitro culture of the venom gland cells of Pseudomyremex triplarinus is effected by removing venom gland tissue from a larva or pupa of P. triplarinus subjecting the tissue to a protease to separate out the cells therein and culturing the resultant cells in an isotonic salin medium comprising carbohydrate, amino acids guanosine 3',5'-cyclic monophosphate and adenosine 3',5'-cyclic monophosphoric acid, at least part of the medium having been conditioned with Spodoptera frugiperda cells. Such in vitro culture provides a practicable route to volume production of the biologically active materials which are useful fortreating the symptoms of rheumatoid arthritis and which the venom gland cells are known to produce.
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