Process for obtaining the globular domains of basement membrane collagen, and immunological determination of basement membrane materials and auto-antibodies

摘要

The spherical basilar memebrane collagen field NCI of people can acquire the first restrictive processing with bacterial collagenase by human object or animal tissue, the breakdown products separating obtained from the non-collagen by the chromatography on weak base exchanger, then subject's collagen breakdown product in the second clostridiopetidase A failure of high temperature is arrived, imrnuglobulin domain NCI is purified by molecular sieve fractionation, with about 170, what the molecular weight of 000 Dalton was determined by ultracentrifugation, the structure of one hexamer, its monomer subunits in relation to molecular weight about 28, 000 Dalton and about 2% a carbohydrate content. It is applicable to determine in the body fluid when utilizing its antibody and for detecting the basilar memebrane collagen directly against its antibody in body fluid.