METHOD OF REGENERATING RICE PLANT FROM PROTOPLASTS

摘要

Method of generating a complete plant of rice from a protoplast thereof. To prepare the protoplast, a callus is derived from an embryo, scutellum, or radicle of a seed of rice. The callus is then cultured in a liquid medium to form a small cell cluster which readily releases a protoplast. The cell cluster is then treated with a protoplast-releasing enzymatic solution to obtain a protoplast. The protoplast is then cultured to form a callus, and the callus is then cultured in a differentiation medium to regenerate a whole plant of rice.