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Method for the specific determination of serum fructosamine content and suitable reagent mixture

机译:血清果糖胺含量和适当试剂混合物的特异性测定方法

摘要

The present invention relates to a method for the specific determination of the serum fructosamine content in blood or blood-derived samples by reaction with a suitable color reagent and measurement of the color change caused thereby. The method according to the invention is characterized in that, before the color reaction, nonspecificly reducing or / and turbidity-causing sample components are removed at an approximately neutral pH value, then a pH value between pH 10 and 12 is set and the color reagent is added. Another object of the invention is a reagent mixture for the specific determination of the serum fructosamine content in blood or blood-derived samples, which is characterized in that it consists of a reagent for the removal of nonspecifically reducing and / or turbidity-causing sample components, a re-buffering reagent with a buffer, the one Has pH in the range of l0.5 to l2.5 and a color reagent for the detection of fructosamine.
机译:本发明涉及一种通过与合适的显色剂反应来具体测定血液或血液来源样品中血清果糖胺含量并测量由此引起的颜色变化的方法。根据本发明的方法的特征在于,在显色反应之前,在大约中性pH值下去除非特异性还原或/和引起浊度的样品组分,然后将pH值设定在pH 10至12之间,并且将显色试剂被添加。本发明的另一个目的是用于特异性测定血液或血液来源的样品中血清果糖胺含量的试剂混合物,其特征在于它由用于去除非特异性降低和/或引起浑浊的样品成分的试剂组成。 ,一种带有缓冲液的重新缓冲试剂,其pH值在l0.5至l2.5范围内,还有一种用于检测果糖胺的显色试剂。

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