Determination of Enantiomeric Compositions of Tryptophan by Chemometric Analysis of the Fluorescence Spectra of Bovine Serum Albumin Receptor-ligand Mixtures

摘要

In this work, a novel method was constructed to determine the enantiomeric composition of tryptophan (Trp) by bovine serum albumin (BSA) based on the fluorescence spectra of the receptor-ligand mixtures coupled with partial least squares (PLS-1) analysis. As a result the enantiomeric composition of Trp was accurately determined.