PRODUCTION OF ERYTHROCYTE DERIVED CU, ZN TYPE SUPEROXIDE DISMUTASE AND CATALASE

摘要

PURPOSE:To recover the title superoxide dismutase and catalase in high purity, in high yield and in a short time, by treating hemolyzed solution with an anion exchanger in the presence of a nonionic surfactant. CONSTITUTION:A blood cell fraction obtained from human blood, etc. is hemolyzed with an equal volume of distilled water to give a hemolyzed solution (A). Then an exchange group such as tertiary amine of diethylamino ethyl group, etc. is bonded to a carrier such as cellulose, etc. to give an anion exchanger (B). Then 0.1-1l component B is added to 1l solution A, which is mixed with 0.05-0.5W/V% nonionic surfactant (C) (e.g. polyoxyethylene decyl ether) equilibratged with a buffer solution at pH 6.5-7 stirred for 1-6hr, filtered under suction to give an exchanger (D) having adsorbed SOD (superoxide dismutase) and catalase. Then the component D is eluted with a buffer solution at pH 6-7 to recover erythrocyte derived Cu, Zn type speroxide dismutase and catalase.