PRODUCTION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 ANTIGEN AND REAGENT FOR DETECTING ANTIBODY INVOLVED USING THE SAME

摘要

PURPOSE:To obtain an antigen, by treating a human immunodeficiency virus type-2, obtained by cultivating a cell produced by the human immunodeficiency virus type-2, by anionic surfactant. CONSTITUTION:To obtain material for human immunodeficiency virus type-2 (HIV-2), HIV-2 is separated from a culture supernatant of the HIV2 produced cell by centrifugal separation or other methods. Then, a buffer containing anionic surfactant such as alkylsodium sulfate is added to a sediment of the HIV-2 to treat. Then, a soluble liquid undergoes a centrifugal separation and the resulting supernatant is used as HIV-2 antigen.