QUANTITATIVE ANALYSIS OF HEMOGLOBIN IN HUMOR

摘要

PURPOSE:To enable the quantitative analysis of hemoglobin in humor safe to the human body by eliminating the toxity of a mutagenic agent, by using tetramethylbenzidine as a color forming reagent. CONSTITUTION:An aqueous hydrogen peroxide solution is added to an acetic acid solution of 3,3'5,5'-tetramethylbenzidine (TMB) while a minute amount (Rpts.vol.) of a body fluid to be examined is added to the resulting solution mixture and, after the obtained reaction mixture is allowed to stand for a definite time at a room temp., a reaction stopping solution such as an acetic acid solution is added to measure the absorbancy Ex of the body fluid to be examined, for example, at 660nm. In the next step, the aqueous hydrogen peroxide solution is added to the acetic acid solution of TMB while a minute amount (Rpts.vol.) of the body fluid to be examined and a hemoglobin standard liqiud (Rpts.vol) having a known concn. Cs are added to the resulting solution mixture and, after, the obtained reaction mixture is allowed to stand for a definite time at a room temp., the reaction stopping solution such as acetic acid is added to measure the absorbancy Es of the standard solution at 660nm. the obtained measured values Ex, Es and the known concn. Cs of the hemoglobin standard solution are substituted for a formula Cx=ExXCs/(Es-Ex) to calculate the hemoglobin conc. Cx in the humor to be examined.