IMMUNOASSAY FOR DEPOSIT OF FAT IN ARTERY OF ATHEROMA ARTERIOSCLEROSIS

摘要

The method of this invention for determining the presence of atherosclerotic plaque antigen in serum comprises contacting the serum with anti-plaque antibody for a time sufficient to permit binding of the atherosclerotic plaque antigen with the anti-plaque antibody, and determining the atherosclerotic anti-idotype antibody, if any binding with the anti-plaque antibody. The anti-plaque antibody can be bound to an insoluble support, contacted with serum to permit atherosclerotic anti-idiotype antibody conjugation with the anti-plaque antibody, and the amount of resultant atherosclerotic anti-idiotype antibody bound to the insoluble support can be determined. Alternatively, a precise amount of atherosclerotic anti-idiotype antibody can be bound to the insoluble support, and a mixture of a predetermined excess amount of labeled anti-plaque antibody and serum can be contacted therewith. The labeled anti-plaque antibody bound to the insoluble support or remaining in the mixture can be determined. Also, a precise amount of anti-plaque antibody can be bound to the insoluble support, and a mixture of a predetermined amount of labeled atherosclerotic anti-idiotype antibody and serum contacted therewith. The labeled atherosclerotic anti-idiotype antibody bound to the insoluble support or remaining in the solution can then be determined.