NORMAL HUMAN CELL PRODUCING PLASMINOGEN ACTIVATION FACTOR AND TRANSFORMED BY INTRODUCTION OF VIRUS GENE AND PREPARATION OF SAID CELL

摘要

PURPOSE:To obtain a cell having high productivity of tissue plasminogen activation factor, by transforming a normal human diploid fibroblast cell IMR90 using a specific method. CONSTITUTION:E.coli HB101 holding a vector pSV3neo containing an SV40T antigen gene is cultured and the obtained vector DNA is linearized with a restriction enzyme. A solution of the linearized vector DNA is added to a cultured suspension of normal human diploid fibroblast cell IMR90 or other normal human cell and the linearized vector DNA is introduced into the cell by electropolation process. A transformed cell capable of producing tissue plasminogen activation factor can be produced by this process.