METHOD FOR CULTIVATING TISSUE OF HYBRID PLANT BODY BELONGING TO GENUS PANAX

摘要

PURPOSE:To obtain young seedling belonging to the genus Panax in a large amount, by forming shoots of a hybrid plant body belonging to the genus Panax and subjecting the above-mentioned shoots to multiple shooting. CONSTITUTION:Sections of roots, stems, flower buds, etc., of hybrids, etc., between Panax ginseng C.A. Meyer and Panax japonicus C.A. Meyer, Panax ginseng and Panax quinquefolium L and Panax japonicus C.A. Meyer and Panax ginseng quinquefolium L. are cultivated in a culture medium, such as Murashige.Skoog culture medium, containing cytokinins, etc., added thereto to form calluses and induce adventitious embryos. Mature embryos of the above-mentioned adventitious embryos are then shot in a redifferentiation culture medium or the immature embryos are subjected to maturity promotion thereof, formation and propagation of secondary embryos and subsequently shot in a redifferentiation culture medium. The resultant shoots are then cultivated in a culture medium containing benzylaminopurine (BAP)-gibberellin (GA) or naphthaleneacetic acid (NAA)-benzylaminopurine (BAP) added thereto while being irradiated with light to form multiple shoots, which are subsequently cultivated in a rooting culture medium containing auxins while being irradiated with light to root and afford the aimed young seedlings.