METHOD OF DIAGNOSING CHLAMIDIA-INDUCED NON-GONOCOCCIC URETHRITIS

摘要

the present invention refers to a method of diagnosis in uretrita negonococica produced by chlamydii.the method consists in that the chance is cropped with secretion uretrala which mix with phosphate buffered saline containing 0.5% bovine albumin, omogenizeaza mixture for 1 h at 37 degree c,in this poliamidice bowls which were treated, in advance, 4 degree c for 18 h with igg horse antichlamydia, washed five times with phosphate buffered saline containing 0.05% tween,treated 15 min at room temperature with 1% bovine albumin solution and then washed with the same buffer, in a report of 0,2 ml of phosphate buffer saline / secretion in mixture.after the mixture is treated at 37 degree c with 1h, 0,1 ml / ball rabbit serum - antichlamydia, crow three times with the same buffer, treat 1 h at 37 degree c, with 0.1 ml / ballanti rabbit igg conjugated alkaline in - phosphatase, washes, and three times with buffer and is treated for 30 min at 37 degree c, with 0.1 ml / ball, solution 0,36% p - nitrofenilfosfat,add an equal volume of solution naoh 2h and read the results.