ABSTRACTA sensitive and specific assay method forluminescent detection of support matrix-boundreporter groups smaller than about 10,000 daltonsin size is disclosed which comprises contacting suchreporter groups with a detector complex which comprisesa first component having a strong and specific affinityfor such reporter groups, and a second component capableof being readily coupled to a bioluminescent or chemi-luminescent light emitting system, thereby to producehigh affinity attachment of said detector complex tothe immobilized reporter group. The amount of bounddetector complex is determined with a luminescencecoupled reaction. The light emitted is quantitatedby means including a luminometer, light sensitive film,or light sensitive charge coupled device; and the amountof such light provides a measure of the reporter groupsbound to the support matrix. In one application, theassay method provides means for monitoring the inter-actions by which the reporter groups are immobilized.Also disclosed are reagent means useful in practicingthe method.
展开▼
