SENSOR FOR MEASUREMENT OF ENZYME HYDROGENATABLE/DEHYDROGENATABLE CHEMICAL SPECIES IN AQUEOUS SOLUTIONS

摘要

An electrode is provided for a single-use sensing device in a clinical chemical analyzer for amperometrically determining the level, in a body fluid, of a chemical species susceptible to dehydrogenation in the presence of a dehydrogenase enzyme. The electrode has a coating comprising the dehydrogenase enzyme, a perfluorosulfonic acid polymer, methylene blue and a cofactor of the group consisting of NAD⁺ and NADP⁺. In a preferred embodiment, the coating also includes a water soluble resinous polymer and a cured aqueous emulsion adhesive.;In another embodiment, the sensor is used to measure a hydrogenatable chemical species, in which case the cofactor is in hydrogenated form. In still another embodiment, the sensor is used to measure a dehydrogenase enzyme, in which case the enzyme is omitted from the coating and a dehydrogenatable chemical species is substituted therefor.