Process for the biotechnological production of rhamnolipids and rhamnolipids with only one beta-hydroxydecanecarboxylic acid moiety in the molecule

摘要

Rhamnolipids with high surface activity are produced micro iologically in high yield per g of dry cell substance using Pseudomonas spec. DSM 2874 in the form of growing, resting and immobilized cell mass in an aqueous medium containing at least one assimilable carbon source at a pH of 6.7 to 7.3 and a temperature of 30 DEG to 37 DEG C. Two new rhamnolipids with only one beta -hydroxydecanoic acid residue in the molecule and defined as alpha -L-rhamnopyranosyl- and 2-O- alpha -L-rhamnopyranosyl- alpha -L-rhamnopyranosyl- beta -hydroxydecanoic acid with a molecular weight of 334 and 480, respectively, are obtained.