Process for reprocessing the solution which is left after the removal of the enzymes upon the enzymatic racematic splitting of an N-acetyl-DL-amino acid in the presence of an L-amino acid acylase

摘要

1. A process for working up the solution - remaining after separation of the enzyme - from the enzymatic racemate resolution of an N-acetyl-DL-amino-carboxylic acid in the presence of an L-amino acid acylase by electrodialysis, characterized in that the electrodialysis unit used consists of a plurality of 3-chamber packs of which each in turn consists of a feed and diluate chamber (1), a concentrate chamber (2) on the cathode side and a concentrate chamber (3) on the anode side, each 3-chamber pack being separated from each of the two adjacent packs by a device (4) in which water is split into OH-ions and H+ ions and in that, on completion of electrodialysis, an aqueous solution of the L-amino acid and at most 50% of the anions originally present in the form of their salts is run off from the diluate chambers (1), an aqueous solution of the cations originally present in the form of hydroxides is run off from the concentrate chambers (2) on the cathode side and an aqueous solution of N-acetyl-D(L)-amino acid and acetic acid in the form of the free acids is run off from the concentrate chambers (3) on the anode side.