METHOD FOR THE REMOVAL OF UNDESIRED CELLS FROM HUMAN LYMPHOCYTE POPULATIONS, APPLICATION OF THE METHOD IN MONOCLONAL ANTIBODY PRODUCTION AND KIT THEREFOR.

摘要

in a process for the in vitro immunization and used to eliminate unwanted cells in populations of cells with human lymphocytes, agents are used which are required to be lysosomotropes in vitro cell populations containing cells cytes humans.a method is also described for the in vitro immunization of lymphocytes for use in the production of human monoclonal antibodies.in this process, lysosomotropes agents are called to act in vitro on human lymphocyte containing cell populations from, in order to enable the removal of cell populations with a negative effect on the in vitro immunization, lymphocytes were immunized. according to the specific antigen in vitro and immortalized.a kit used in the immunization of lymphocytes in vitro and used for the production of human monoclonal antibodies is also described. it contains as active component and a container of a container of lymphokines lysosomotropes agents.