Genetic design of human insulin using genetic engineering techniques and manufacture of human insulin using it

摘要

In the method, using the synthetic method of solid phosphite the oligonucleotide coding for human insulin A and B was synthesized. From the synthesized oligonucleotides, the genes for A and B chain of insulin was constructed by the shot-gun method. After the E. coli plasmid, pUC19 was opened by the restriction enzyme Sph I and BamH I, and the above constructed gene was inserted by the reaction of DNA ligase. The inserted plasmid was called pSY A and pSY B. The base sequence of pSY A and pSY B was identified by the Sanger's method.