Selective growth medium for isolation of Mobiluncus from vaginal fluid

摘要

A selective growth medium for the isolation of Mobiluncus includes a medium base including an aqueous preparation of a gelling agent and a nutrient broth effective for culturing Mobiluncus. The selective growth medium of the invention further includes a first antimicrobial agent in effective antimicrobial amounts, the antimicrobial agent being selected from the group consisting of colistin, nalidixic acid, and combinations of colistin and nalidixic acid, the colistin having a concentration less than 32 micrograms/ml and the nalidixic acid having a concentration less than 100 micrograms/ml. Tinidazole is included as a second antimicrobial agent in effective, antimicrobial amounts not greater than 1.0 micrograms/ml. Nile Blue A is also included in Gardnerella Vaginalis inhibiting concentrations.