Selective culture medium composition for pure acido-halophilic microorganisms lived in semi-fluidal conditions and a manufacturing method of selective culture medium

摘要

PURPOSE: Provided are a selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions and a manufacturing method the selection medium using the same composition, thereby culturing the microorganisms with higher growth rate than in an MRS medium or a YM medium. CONSTITUTION: The selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions comprises 3 to 7 g/l of yeast extract, 1.5 to 4 g/l of malt extract, 1.5 to 4 g/l of meat extract, 8 to 12 g/l of tryptone or peptone, 15 to 25 g/l of glucose or lactose, 0.5 to 1.5 g/l of polysorbate 80, 1 to 3 g/l of citric acid or malic acid, 25 to 35 g/l of sodium chloride, and 7 to 9 g/l of agar, wherein 25 to 35 ml/l of glycerol is optionally added to the composition. The method for producing the selective culture medium comprises the steps of: mixing yeast extract, malt extract, meat extract, tryptone or peptone, glucose or lactose, polysorbate 80, citric acid or malic acid, sodium chloride, and agar and inserting the mixture into a flask; adding water into the mixture; slightly heating the mixture; adding glycerol into the heated mixture; and sealing the flask and sterilizing the mixture.