MONOCLONAL ANTIBODY AGAINST MYOCARDIAL MYOSIN HEAVY CHAIN

摘要

PURPOSE:To enable obtaining of pathological diagnosis by selecting an IgG having specificity for human myocardial myosin heavy chain alpha type without recognizing human myocardial myosin heavy chain beta type and human skeletal muscle. CONSTITUTION:A mouse, etc., is immunized with myocardial myosin, derived from cattle, etc., of the immunochemically same type as that of human myocardial myosin heavy chain alpha (beta) type to provide a cell capable of producing an antibody (A). A myelomatous cell (B) prepared from a rat, etc., is mixed with the cell (A) at 1:(4-10) mixing ratio and subjected to cell fusion in the coexistence of PEG to afford a hybridoma (C). The resultant component (C) is then searched for by an enzyme immunoassay to provide a hydridoma capable of producing an antihuman myocardial myosin heavy chain alpha type antibody and hybridoma (D) capable of producing an antihuman myocardial myosin heavy chain beta type antibody. The component (D) is subsequently cloned by a limiting dilution method, etc., to afford a hybridoma (E) capable of producing an antibody, which is then cultured in a bovine fetal blood serum-containing culture medium, etc., to produce a monoclonal antibody against the myocardial myosin heavy chain in the culture supernatant liquid.