PURPOSE:To dispense with the dilution of serum and to obtain a measured value within a short time with good accuracy by using two or more kinds of anti-human apo B monoclonal antibodies having different antigenic determinants to be recognized. CONSTITUTION:The antigen-antibody reaction with serum is performed using two or more kinds of anti-human apo B monoclonal antibodies having different antigenic determinants to be recognized and the turbidity due to the generated antigen-antibody composite is measured spectroscopically. By this method, the dilution of serum becomes unnecessary and measurement can be performed within an extremely short time. For example, when four kinds of monoclonal antibodies are used in combination, antigen-antibody reaction reaches a plateau after about 9min. Further, by this method, serum apo B can be accurately measured up to concentration of at least about 230mg/dl.
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