首页> 外国专利> STRUCTURAL GENE OF CELL MEMBRANE BONDING TYPE ALDEHYDE DEHYDROGENASE, PLASMID CONTAINING THE SAME, TRANSFORMED ACETIC ACID BACTERIUM AND METHOD FOR FERMENTING ACETIC ACID

STRUCTURAL GENE OF CELL MEMBRANE BONDING TYPE ALDEHYDE DEHYDROGENASE, PLASMID CONTAINING THE SAME, TRANSFORMED ACETIC ACID BACTERIUM AND METHOD FOR FERMENTING ACETIC ACID

机译:细胞膜键合型乙醛脱氢酶的结构基因,包含相同质体的质粒,转化的乙酸细菌及其发酵方法

摘要

PURPOSE:To enhance content of an enzyme in a cell as well as efficiency of fermentation and enable efficient production of a desired acid by isolating a structural gene of cell membrane bonding type aldehyde dehydrogenase and integrating the isolated gene into a plasmid. CONSTITUTION:A microorganism belonging to the genus Acetobacter or Gluconobacter is transformed by a plasmid containing a structural gene of a cell membrane bonding type aldehyde dehydrogenase derived in a microorganism of the genus Acetobacter and having about 3.6 kilo base molecular size and fermentation is carried out using the transformed microorganism to provide the acid, especially acetic acid. The map of restriction enzyme of the structural gene is shown in the figure.
机译:目的:通过分离细胞膜结合型醛脱氢酶的结构基因并将分离的基因整合到质粒中,以提高细胞中酶的含量以及发酵效率,并能有效产生所需的酸。组成:醋杆菌属或葡糖杆菌属的微生物通过质粒转化,该质粒含有醋杆菌属微生物衍生的细胞膜键合型醛脱氢酶的结构基因,分子大小约为3.6千克,并使用转化的微生物以提供酸,尤其是乙酸。该结构基因的限制酶图谱如图所示。

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