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GENETIC ENGINEERING TECHNIQUE FOR PRODUCING NEW, HUMAN INTERLEUKIN-2 PROTEIN
GENETIC ENGINEERING TECHNIQUE FOR PRODUCING NEW, HUMAN INTERLEUKIN-2 PROTEIN
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机译:产生新的人类白介素2蛋白的基因工程技术
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摘要
A substantially pure non-glycosylated human interleukin-2 protein (I) with a specific activity of at least 10,000 U/mg is new. (I) has an amino acid sequence as shown in the figure, where X = Met or H. A bacterial transformant contg. a DNA sequence coding for human interleukin-2, esp. E. coli DHI/pTF4 (IFO 14299, FERM BP628), is cultured to accumulate (I) in the broth, which is purified by hydrophobic column chromatography, esp. reverse-phase HPLC.
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