Appts. for in-vitro determn. of receptors in biological material - has twin layers of material incubated with soln. of ligands marked by short-lived isotopes

摘要

The appts. used comprises an incubation container (1), the limiting surfaces of which comprise a base (1a) and four side walls (1b-e). The container is divided by intermediary walls (2) into several chambers, and pref. also into several heat exchanging chambers (4). Each heat exchanging chamber (4) is arranged adjacent to one or two incubation chambers (3), each of which has an aperture (5) in its lower part acting as inlet and outlet. Each heat exchanging chamber has apertures (6) (7) in its upper and lower parts for input and outlet of heat exchanging medium. The thickness of the cerebral tissue sample used is pref. 80 micrometres, and its is arranged on a carrier which is then inserted into the incubation container, into which a soln. contg. the marked ligands in a specific concn. is also introduced. Incubation takes place at a constant temp. selected in the range of 0 - 40 deg.C., so that the ligand binding properties are optimised and the receptors with the ligands are pr operly saturated. After removal of the incubation soln., the sample is rinsed several times with a buffer soln. and possibly with distilled water, and the radiation from it is recorded.