specific polypeptide producing hybridzellinien, available from primaerzellen who spontaneously to expres sion of this polypeptids are able or a similar polypeptids, procedures to receive these hybrid cells and their use for the production of this polypeptids.

摘要

Hybrid cells transformed or transformable by a cloned DNA sequence containing the sequence coding for a determined polypeptide, for example an immunogene polypeptide having vaccination properties to hepatitis B. They are characterized in that they contain on one hand at least a portion of the genotype of a primary cell lineage which are naturally favorable to the expression of said DNA sequence and on the other hand a genetic marker which enable them to grow in a selective medium or containing an active principle which is normally lethal for the cells from which the hybrid is issued, but unactivable by the polypeptide expressed by said genetic marker.