ANAPLASMA MARGINALE SUBUNIT ANTIGEN FOR VACCINATION AND DIAGNOSIS

摘要

Abstract of the DisclosureSubstantially pure antigenic surface proteins of Anaplasma marginalehave been identified, and are capable of inducing immune responses inruminants which neutralizes virulent Anaplasma marginale. The antigenicsurface proteins have one or more components having a molecular weight ofabout 105,000 daltons, 86,000 daltons, 61,000 daltons, 36,000 daltons,31,000 daltons, or 15,000 daltons, and can be purified by an immunoaffinitychromotography process comprising the steps of disrupting Anaplasmamarginal initial bodies by treatment with a detergent, passing thedisrupted initial bodies over a chromotogrnphy column comprising aninsoluble matrix coupled to monoclonal antibodies against a determinant onsaid antigenic surface protein to selectively bind said antigenic surfaceprotein to said monoclonal antibodies and recovering the bound substantiallypure antigenic surface protein from said insoluble matrix. The antigenshave further utility in diagnostic tests for anaplasmosis. They can besynthesized by polypeptide procedures or by genetic engineering.