首页>
外国专利>
CYTOCHROME P-450 HFLA PROTEIN, ITS DNA SEQUENCE AND A PROCESS FOR PRODUCING SAID PROTEIN
CYTOCHROME P-450 HFLA PROTEIN, ITS DNA SEQUENCE AND A PROCESS FOR PRODUCING SAID PROTEIN
展开▼
机译:细胞色素P-450 HFLA蛋白质,其DNA序列和生产唾液蛋白质的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE:To make it possible to obtain the title protein in large quantities without containing a human-derived other protein by culturing a recombinant host cell in a culture medium. CONSTITUTION:A human fetal lever is purified by homogenizing in a buffer to afford a cytochrome P-450HFLa protein (A) having about 51000 molecular weight. Then a rabbit, etc., is immunized with the ingredient A to give an anti-P-450HFLa antibody (B). On the other hand, a fetal lever is treated with guanidinethiocyanate to extract total RNA, which is then chromatographed to afford mRNA (C). Then the ingredient C is translated to a protein and subjected to screening with the ingredient B to provide the desired mRNA fraction (D). Then the ingredient D is synthesized to a single-stranded cDNA, which is then treated with ligase to give a double-stranded cDNA (E). Then a recombinant DNA obtained by adding a linker to the ingredient E and introducing into a plasmid is introduced into escherichia coli and the resultant transformed strain is cultured in a culture medium and DNA sequence such as DNA expressed by formula I is expressed to produce cytochrome P-450HFLa protein containing an amino acid sequence expressed by formula II.
展开▼