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A DNA DIAGNOSTIC TEST USING AN EXONUCLEASE ACTIVITY
A DNA DIAGNOSTIC TEST USING AN EXONUCLEASE ACTIVITY
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机译:利用核酸外切酶活性进行DNA诊断试验
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摘要
A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nuclotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence or absence of the test nucleotide then may be established by determining whether the label is preserved or lost following the reaction.
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