DNA POLYMERASE VARIANTS WITH REDUCED EXONUCLEASE ACTIVITY AND USES THEREOF

摘要

Compositions and methods are described to modify Family B DNA polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available "exo^-" polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic DNA polymerase.