Process for mammalian cell suspension cultivation device therefor and use in the production of proteins

摘要

The present invention relates to a method for the cultivation of mammalian cells in suspension culture the medium exchange is performed by ultrafiltration. The culture medium is withdrawn via first eie microporous membrane from the reactor to obtain a cell-free medium. This medium is supplied to a Ultrafiltrationsmem bran and ultrafiltered. The ingredients and the niedermole-molecular metabolic products contained tend filtrate is discarded and the concentrate fed to the reactor. From a medium reservoir to the reactor according to the amount of filtrate is fed fresh medium.