Reagent composition for immunoassay and immunoassay using the same

摘要

@ immunoassay which can be performed within a short period of time and a reagent composition for such immunoassay. The reagent composition contains complement and cells which contain quantitatively determinable substance therein. Antibody or antigen corresponding to antigen or antibody to be determined in a sample is bound on the outer surfaces of the cell membranes, which can be lysed by the complement upon activation thereof. In the immunoassay, a sample containing antigen or antibody to be determined is mixed with the reagent composition. The complement is activated by the resultant antigen-antibody complex. The cell membranes are lysed by the complex and the determinable substance in the cells is liberated from the cells. The amount of the liberated substance is determined.