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PRODUCTION OF IMMUNOASSAY REAGENT, IMMUNOASSAY REAGENT, IMMUNOASSAY AND PRESERVATION OF IMMUNOASSAY REAGENT
PRODUCTION OF IMMUNOASSAY REAGENT, IMMUNOASSAY REAGENT, IMMUNOASSAY AND PRESERVATION OF IMMUNOASSAY REAGENT
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机译:免疫测定试剂的生产,免疫测定试剂,免疫测定和免疫测定试剂的保存
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摘要
PROBLEM TO BE SOLVED: To obtain an immunoassay reagent prevented from the lowering of reactivity and non-special autoagglutination of an antigen or an antibody by supporting the antigen or the antibody on an insoluble carrier in the presence of a protein folding factor. ;SOLUTION: For example, molecular chaperon and peptidyl propyl cis-trans isomerase (PPI ase) having action for keeping a three-dimensional structure of an antigen or an antibody or action of quickly restoring a structural change are pref. used as a protein folding factor. As an insoluble carrier, from a reason capable of shortening an examination time and saving labor, for example, polystyrenic or synthetic rubber type latex is especially pref. used. For example, an immunoassay reagent is produced by bringing an antigen or antibody soln. prepared by diluting an antigen or an antibody with a pref. buffer soln. and the insoluble carrier into contact with each other in an aq. medium containing the protein folding factor to incubate them for a predetermined time to fix the antigen or the antibody to the insoluble carrier.;COPYRIGHT: (C)1999,JPO
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