Method and apparatus for bone histomorphometry

摘要

A method for bone histomorphometry by using a non-decalcified bone specimen, comprising the steps of:a) obtaining two pictures A and B by recording different chromatic light transmitted through a same region of said non-decalcified bone specimen or by recording different chromatic fluorescene from the region of said non-decalcified bone specimen;b) detecting the strength (brightness) L_An and L_Bn of the corresponding image element (pixels) An and Bn of said two pictures A and B;c) determining the ratio L_An/L_Bn;d) performing the steps b) and c) on all corresponding image element of said two pictures A and B; ande) with regard to the ratio L_An/L_Bn, calculating and expressing areas of three components of bone, said three components of bone being a calcified bone area, an osteoid area, and a bone marrow area, in said non-decalcified bone specimen.