Method and apparatus for bone histomorphometry

摘要

A method for bone histomorphometry by using a non-decalcified bone specimen, comprising the steps of: (a) obtaining two pictures A and B by recording different chromatic light transmitted through a same region of said non-decalcified bone specimen or by recording different chromatic fluorescence from the region of said non-decalcified bone specimen; (b) detecting the strength (brightness) L.sub.An and L.sub.Bn of the corresponding image elements (pixels) An and Bn of said two pictures A and B; (c) determining the ratio L.sub.An /L.sub.Bn ; (d) performing the steps (b) and (c) on all corresponding image elements of said two pictures A and B; and (e) with regard to the ratio L.sub.An /L. sub.Bn, calculating and expressing areas of three components of bone, said three components of bone being a calcified bone area, an osteoid area, and a bone marrow area, in said non-decalcified bone specimen.