process for producing protein from a microorganism, microorganism for use in such process and their productionvectors for use in the production and protein produced by the microorganism and the microorganism derived transformed cultures.

摘要

The invention discloses an improved method and cloning vectors useful for the production of cloned heterologous gene products in B. subtilis. Use of the method and vectors allows the host to produce the heterologous gene product as a single unfused peptide having no extraneous amino acids attached that will accumulate within transformed host organisms. The method comprises providing growth conditions in a growth media for B. subtilis bacteria containing plasmids capable of replication in B. subtilis or capable of integrating into the bacterial chromosome of B. subtilis, said plasmids having a heterologous gene therein coding for the predetermined protein and capable of expession in B. subtilis, said heterologous gene including a translational initiation codon sequence and being under the control of operable transcriptional and translational regulatory signals including operator, promoter and ribosomal binding site sequences, said operable transcriptional and translational regulatory signals being indigenous to a source other than said heterologous gene, and there being no translational initiation codon sequences prior to said heterologous gene, and optionally recovering the resulting predetermined protein.