Method for determining the presence of specific igg in the human sera

摘要

The invention consists in a process for determining the presence of a specific IgG in human sera, characterised in that it comprises the following steps: binding of previously purified complex protein extracts upon a solid support, followed by incubation of the serum sample with the said bound protein extracts: washing non-specific proteins; incubation with anti-IgG monoclonal antibody labelled with 125I or with an enzyme; subsequent washing of the solid support with the bound products and determination of the radioactivity or enzymatic activity incorporated into the solid support.