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Expression of non-structural protein of Japanese encephalitis virus in insect cells

机译:乙型脑炎病毒非结构蛋白在昆虫细胞中的表达

摘要

Recombinant Baculovirus in which the cDNA encoding non-structural protein derived from Japanese encephalitis virus is integrated into the genome region non-essential to proliferation of Baculovirus under control of the promoter is infected to insect cells such as Sf9 cells, derived from Spodoptera frugiperda and the infected cells are cultured to express non- structural protein of Japanese encephalitis. This non-structural protein is useful as vaccines or diagnostics.
机译:重组杆状病毒被编码到昆虫细胞如Sf9细胞中,该重组杆状病毒将编码来自日本脑炎病毒的非结构蛋白的cDNA整合到在启动子控制下对杆状病毒的增殖非必需的基因组区域中。培养感染的细胞以表达日本脑炎的非结构蛋白。这种非结构蛋白可用作疫苗或诊断试剂。

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