Method for decontamination of mycoplasma-infected cell cultures

摘要

A method for decontaminating cell cultures comprising contacting cell cultures with quinolone- and 1,8-naphthyridonecarboxylic acids of the formula (I) ##STR1## in which R.sup.1 represents methyl, ethyl, propyl, isopropyl, cyclopropyl, vinyl, 2-hydroxyethyl, 2- fluorethyl, methoxy, amino, methylamino, dimethylamino, ethylamino, phenyl, 4-fluorophenyl or 2,4-difluorophenyl,PPR.sup.2 represents hydrogen, alkyl having 1 to 4 carbon atoms, or (5-methyl-2-oxo- 1,3-dioxol-4-yl)-methyl, PPR.sup.3 represents methyl or a cyclic amino group such as ##STR2## in which R.sup.4 represents hydrogen, alkyl having 1 to 4 carbon atoms, 2-hydroxyethyl, allyl, propargyl, 2- oxopropyl, 3-oxobutyl, phenacyl, formyl, CFCl.sub.2 --S--, CFCl.sub.2 -- SO.sub.2 --, CH.sub.3 O-- CO--S--, benzyl, 4-aminobenzyl or ##STR3## R. sup.5 represents hydrogen or methyl, R.sup.6 represents hydrogen, alkyl having 1 to 4 carbon atoms, phenyl or benzyloxomethyl,PPR. sup.7 represents hydrogen, amino, methylamino, ethylamino, aminomethyl, methylaminomethyl, ethylaminomethyl, dimethylaminomethyl, hydroxyl, or hydroxymethyl, PPR.sup.8 represents hydrogen, methyl, ethyl or chlorine PPX represents fluorine, chlorine or nitro andP PA represents N or C-R.sup.9, in whichP PR.sup.9 represents hydrogen, halogen such as fluorine or chlorine, methyl or nitro, or can also, together with R.sup.1, form a bridge of the structure --O--CH.sub.2 --CH--CH.sub.3, --S--CH.sub.2 --CH-- CH.sub.3 or --CH.sub.2 - -CH.sub.2 --CH--CH.sub.3.